In vivo investigation of open-pored magnesium scaffolds LAE442 with different coatings in an open wedge defect

authored by: Marlene Schmidt, Anja Christina Waselau, Franziska Feichtner, Stefan Julmi, Christian Klose, Hans Jürgen Maier, Peter Wriggers, Andrea Meyer-Lindenberg
Abstract: The magnesium alloy LAE442 showed promising results as a bone substitute in numerous studies in non-weight bearing bone defects. This study aimed to investigate the in vivo behavior of wedge-shaped open-pored LAE442 scaffolds modified with two different coatings (magnesium fluoride (MgF₂, group 1)) or magnesium fluoride/calcium phosphate (MgF₂/CaP, group 2)) in a partial weight-bearing rabbit tibia defect model. The implantation of the scaffolds was performed as an open wedge corrective osteotomy in the tibia of 40 rabbits and followed for observation periods of 6, 12, 24, and 36 weeks. Radiological and microcomputed tomographic examinations were performed in vivo. X-ray microscopic, histological, histomorphometric, and SEM/EDS analyses were performed at the end of each time period. µCT measurements and X-ray microscopy showed a slight decrease in volume and density of the scaffolds of both coatings. Histologically, endosteal and periosteal callus formation with good bridging and stabilization of the osteotomy gap and ingrowth of bone into the scaffold was seen. The MgF₂ coating favored better bridging of the osteotomy gap and more bone-scaffold contacts, especially at later examination time points. Overall, the scaffolds of both coatings met the requirement to withstand the loads after an open wedge corrective osteotomy of the proximal rabbit tibia. However, in addition to the inhomogeneous degradation behavior of individual scaffolds, an accumulation of gas appeared, so the scaffold material should be revised again regarding size dimension and composition.
Organisation(s): Institute of Materials Science
Institute of Continuum Mechanics
External Organisation(s): Ludwig-Maximilians-Universität München (LMU)
Type: Article
Journal: Journal of Applied Biomaterials and Functional Materials
Volume: 20
ISSN: 2280-8000
Publication date: 2022
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Biophysics, Bioengineering, Biomaterials, Biomedical Engineering
Electronic version(s): https://doi.org/10.1177/22808000221142679 (Access: Open)
: Details in the research portal "Research@Leibniz University"

BibTeX

@article{5dd5fc47fd774508a708bf9244117390,
title = "In vivo investigation of open-pored magnesium scaffolds LAE442 with different coatings in an open wedge defect",
abstract = "The magnesium alloy LAE442 showed promising results as a bone substitute in numerous studies in non-weight bearing bone defects. This study aimed to investigate the in vivo behavior of wedge-shaped open-pored LAE442 scaffolds modified with two different coatings (magnesium fluoride (MgF2, group 1)) or magnesium fluoride/calcium phosphate (MgF2/CaP, group 2)) in a partial weight-bearing rabbit tibia defect model. The implantation of the scaffolds was performed as an open wedge corrective osteotomy in the tibia of 40 rabbits and followed for observation periods of 6, 12, 24, and 36 weeks. Radiological and microcomputed tomographic examinations were performed in vivo. X-ray microscopic, histological, histomorphometric, and SEM/EDS analyses were performed at the end of each time period. µCT measurements and X-ray microscopy showed a slight decrease in volume and density of the scaffolds of both coatings. Histologically, endosteal and periosteal callus formation with good bridging and stabilization of the osteotomy gap and ingrowth of bone into the scaffold was seen. The MgF2 coating favored better bridging of the osteotomy gap and more bone-scaffold contacts, especially at later examination time points. Overall, the scaffolds of both coatings met the requirement to withstand the loads after an open wedge corrective osteotomy of the proximal rabbit tibia. However, in addition to the inhomogeneous degradation behavior of individual scaffolds, an accumulation of gas appeared, so the scaffold material should be revised again regarding size dimension and composition.",
keywords = "biodegradation, coating, Magnesium alloy, osseointegration, porous scaffold",
author = "Marlene Schmidt and Waselau, {Anja Christina} and Franziska Feichtner and Stefan Julmi and Christian Klose and Maier, {Hans J{\"u}rgen} and Peter Wriggers and Andrea Meyer-Lindenberg",
note = "Funding Information: The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This project was financially supported by the German Research Foundation (DFG) within the project “Interfacial effects and ingrowth behavior of magnesium sponges as a bioresorbable bone graft substitute” (Grant No. 271761343). ",
year = "2022",
doi = "10.1177/22808000221142679",
language = "English",
volume = "20",
journal = "Journal of Applied Biomaterials and Functional Materials",
issn = "2280-8000",
publisher = "SAGE Publications Inc.",
}